Protective Effect of Anar-5 Herbal Treatment on Experimental Chronic Atrophic Gastritis in Wistar Rats

  • Erdenetsogt Dungubat National University of Medical Sciences (MNUMS)
  • Uranzaya Dashzeveg Institute of Traditional Medicine and Technology of Mongolia
  • Dejidmaa Buyantogtokh Institute of Traditional Medicine and Technology of Mongolia
  • Chimedragchaa Chimedtseren Institute of Traditional Medicine and Technology of Mongolia
  • Bayarmaa Enkhba
  • Batkhuyag Purevjav Mongolian National University of Medical Science

Abstract

Background: Chronic gastritis is a slowly progressive disease. It includes atrophy of gastric mucosa, impairment of epithelial regeneration, formation of lymphoid tissue/germinal center, loss of gastric secretion and movement. The Mongolian traditional medicine used Anar-5 recipes for the treatment of stomachache, emesis, and improvement of gastricdigestion. Based on these observations, we implemented a rat model of Anar-5 treatment in artificial chronic gastritis in order to elucidate its potential protective mechanisms scientifically.
Methods and material: We established an experimental rat model of chronic gastritis by use of ammonia water. We divided the rat cohorts in an Anar-5–treated test group, an untreated cohort, and a control group. The untreated group was fed with 0.1% ammonia water and the treated group with both 0.1% ammonia water and administered Anar-5 100 mg/kg/day for 6 weeks. Gastric lesions were evaluated microscopically. The Prostaglandin E2 levels, cyclooxygenase COX-2 expression and the cellular proliferation marker Ki67 were in addition assessed.
Results: The Anar-5 cohort displayed with an increased thickness of the antrum mucosa, number (р˂0.05) and regeneration zones of gastric mucous epithelial cells when compared to the results of the untreated cohort (693.1±63.8 μm versus 429.6±43.5 μm). The untreated cohort displayed with decreased PGE2 levels (14.8±0.62 ng/dl) when compared to those of the control group and Anar-5 cohort (19.5±1.22 ng/dl and 8.7+0.32 g/dl protein, respectively). The Ki67-associated proliferation rate of the antrum mucosa was enhanced and measured 19.75% in the untreated cohort in comparison to a proliferation rate of 6.58% in the Anar-5 cohort.
Conclusion: The data of our rat experiment indicate that a contemporary application of Anar-5 herbs acts as a gastric mucosal protective agent. In addition it induces an overexpression of COX-2 and maintenance of the PGE2 lev

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Author Biography

Erdenetsogt Dungubat, National University of Medical Sciences (MNUMS)

Department of Pathology, School of Pharmacy and Biomedicine University Mongolian National University of Medical Sciences (MNUMS) Street S.Zorig street-3, Ulaanbaatar 14210, MONGOLIA

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Published
2018-01-23
How to Cite
DUNGUBAT, Erdenetsogt et al. Protective Effect of Anar-5 Herbal Treatment on Experimental Chronic Atrophic Gastritis in Wistar Rats. Diagnostic Pathology, [S.l.], v. 4, n. 1, jan. 2018. ISSN 2364-4893. Available at: <http://www.diagnosticpathology.eu/content/index.php/dpath/article/view/258>. Date accessed: 20 jan. 2020. doi: https://doi.org/10.17629/www.diagnosticpathology.eu-2018-4:258.
Section
Research

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